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Flowering


The flowering plants (angiosperms) go through a phase of vegetative growth - producing more stems and leaves - and a flowering phase where they produce the organs for sexual reproduction.

Vegetative growth of the above-ground part of the plant - the shoot - occurs at the apical meristem. This is a mass of undifferentiated cells at the tip of the stem. Mitosis of these cells produces cells that differentiate to form

Discussion of vegetative plant growth

The signal to flower

Flowering involves the conversion of the apical meristem into a floral meristem, from which all the parts of the flower will be produced. Signals that change the fate of the apical meristem include:

Photoperiod is detected in the leaves. The cocklebur, drawn here, needs at least 8.5 hours of darkness in order to flower. Even if only a part of one leaf is exposed to the correct photoperiod, the entire plant will bloom (middle figure).

The leaves produce a chemical signal - called florigen - that is transmitted to the apical meristems to start their conversion into floral meristems. The right-hand drawing shows that grafting a cocklebur (B) that receives the required period of darkness to one (A) that does not causes flowering in both. Evidently the florigen signal passes from B to A through their connected vascular systems.

Link to a discussion of photoperiodism.

The chemical nature of florigen has been sought for decades. The most recent evidence suggests that it is a zinc-finger transcription factor.

Converting the apical meristem to a floral meristem

The protein encoded by the Arabidopsis gene LEAFY (LFY) appears to be the master switch that switches the fate of the apical meristem from vegetative growth to forming a floral meristem. The LEAFY protein is transcription factor.

Structure of the Flower

The floral meristem differentiates into four concentric groups of cells that form the four parts of the flower.
  1. The cells in whorl 1 develop into a whorl of sepals. These form at the lowest level. Collectively they make up the calyx.
  2. Whorl 2 forms above the calyx, forming the petals. Collectively these make up the corolla of the flower (the part that most ornamentals are grown for).
  3. Whorl 3 develops into the stamens, the male reproductive organs.
  4. The innermost whorl, 4, forms carpels, the female reproductive organs. Carpels often fuse to form a single structure, which some botanists call the pistil.

What triggers the various parts of the floral meristem to enter one or another of these four developmental pathways?

The ABC Model of Flower Development

Genetic analysis of mutants - especially those found in the dicots Arabidopsis thaliana and in the snapdragon (Antirrhinum) support the ABC model of flowering. This model postulates a group of genes that encode the transcription factors needed to turn on the genes for sepal, petal, etc. development. The "master switches" fall into 3 groups: A, B, and C. These are the rules:
Examples of A, B, and C group genes involved in flowering.
These have been identified in Arabidopsis thaliana.
A group APETALA1 (AP1) and
APETALA2 (AP2)
B group APETALA3 (AP3) and
PISTILLATA (PI)
C group AGAMOUS (AG)

The LEAFY protein, in addition to its role in making the floral meristem, plays a major role in turning on the A, B, and C group genes in the appropriate locations.

If LEAFY alone is sufficient to turn on AP1, why isn't AP1 expressed in all four whorls?

The answer: AGAMOUS blocks the expression of AP1, so any cell expressing AGAMOUS cannot express AP1.

In fact, the antagonism is reciprocal: AP2 in whorls 1 and 2 (A group) inhibits AGAMOUS so the gene expression in whorls 3 and 4 remains distinct from that in whorls 1 and 2.

The proteins encoded by APETALA3 and PISTILLATA (Group B) form a heterodimer that binds to

Aided by a fourth transcription factor encoded by the gene SEPALLATA3, these quaternary complexes bind to specific sequences of DNA turning on the expression of the various genes needed to form whorls 2 and 3. Further research may reveal similar behavior for the other genes.

So formation of a flower requires a cascade of sequential gene activity that gradually converts a mass of undifferentiated cells (the apical meristem) into the parts of a flower. The genes encode transcription factors that act as master switches, turning on (or off) downstream genes that ultimately make each part of the flower in its appropriate location.

This same strategy of genetic control of developmental pathways is seen in animal development. Try this link to see some examples in Drosophila.

Recommended reading: The Genetics of Flower Development by Elliot M. Meyerowitz (in whose lab many of these discoveries were made). It was published in the November 1994 issue of Scientific American.

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2 February 2001